Tools:
Basic Local Alignment Search Tool
This tool from IDT DNA is a very handy tool for calculating Melting Temperatures for use with various kinds of PCR. Their version is especially effective because it allows you to account for oligo, sodium, magnesium, and dNTP concentrations, which can affect your calculation quite drastically.
NEB cutter is a versatile program that quickly calculates which restriction enzymes will cut your entered DNA sequence and how many times they will cut. The program even has options that account for restriction enzymes outside of the NEB catalog. If you are doing a double digest, this link here will quickly calculate the best buffer for your digest.
This massive list of tools applies most directly to proteomics, but there are many helpful tools for geneticists as well. If you are looking for a bioinformatics tool and need a good place to start, look no further.
Saccharomyces Genome Database (SGD)
A must for any lab working with the common baker’s yeast. Includes virtually anything you would want to know from a molecular or genetic perspective, including BLAST.
Massive genomic database of worm-based parasites with both flatworms and nematodes, schistosomes included. Has both nucleotide and protein sequence searches, comparative genomic data, and semi-automatic annotation
Schistosoma mansoni Gene Database
Similar to the SGD above, this is a historic BLAST database for S. mansoni. It is active but no longer maintained.
Vendors:
Protocols:
This section currently under construction. Please check back later.
Other:
Case Western Reserve Biology Department
This link will take you to the department home page. To visit Dr. Jolly’s personal page, click here.